Fecha/hora: 14/05/2024 03:18:31

Biblioteca(s): 	INIA Tacuarembó.	
Fecha corriente: 	13/01/2020	
Fecha de la última actualización: 	01/06/2020	
Autoria: 	OBERTI, H.; DALLA RIZZA, M.; REYNO, R.; MURCHIO, S.; ALTIER, N.; ABREO, E.	
Afiliación: 	HÉCTOR OBERTI RVAROLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Diversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes.	
Año de publicación: 	2020	
Fuente/Imprenta: 	Mycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827	
DOI: 	10.1080/00275514.2019.1694827	
Idioma: 	Inglés	
Notas: 	Article history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay (grant numbers SA-24, BT-19).	
Contenido: 	Claviceps species affecting Paspalum spp. are a serious problem, as they infect forage grasses such as Paspalum dilatatum and P. plicatulum, producing the ergot disease. The ascomycete C. paspali is known to be the pathogen responsible for this disease in both grasses. This fungus produces alkaloids, including ergot alkaloids and indole-diterpenes, that have potent neurotropic activities in mammals. A total of 32 isolates from Uruguay were obtained from infected P. dilatatum and P. plicatulum. Isolates were phylogenetically identified using partial sequences of the genes coding for the second largest subunit of RNA polymerase subunit II (RPB2), translation elongation factor 1-? (TEF1), ?-tubulin (TUB2), and the nuc rDNA 28S subunit (28S). Isolates were also genotyped by randomly amplified polymorphic DNA (RAPD) and presence of genes within the ergot alkaloid (EAS) and indole-diterpene (IDT) biosynthetic gene clusters. This study represents the first genetic characterization of several isolates of C. paspali. The results from this study provide insight into the genetic and genotypic diversity of Claviceps paspali present in P. dilatatum and suggest that isolates from P. plicatulum could be considered an ecological subspecies or specialized variant of C. paspali. Some of these isolates show hypothetical alkaloid genotypes never reported before.	
Palabras-Clave: 	ERGOT; FINGERPRINTING; HOST SPECIATION; PASPALUM; PHYLOGENY; STAGGER.	
Categoría del tema: 	A50 Investigación agraria	
Registro original: 	INIA Tacuarembó (TBO)	
Ejemplares: 	TBO - (103133-1) - INI - AP - DD -  -  -  - DIS